Use of free testosterone testing is not recommended for hypogonadism or hyperandrogenism diagnosis.

Type of practice

Laboratory tests

Topic Area


Testosterone is the body’s most important androgen. The measure of its concentration is indicated in numerous clinical conditions in both males and females. However, there are analytical problems such as to make the interpretation and use of the free quota dosage not always transparent; the reference method, equilibrium dialysis, is not feasible on a large scale. It is therefore preferable not to propose this type of evaluation but to rely on total testosterone.


1. Miller KK, Rosner W, Lee H, Hier J, Sesmilo G, Schoenfeld D, Neubauer G, Klibanski A. Measurement of free testosterone in normal women and women with androgen deficiency: comparison of methods. J Clin Endocrinol Metab. 2004 Feb;89(2):525-33.
2. Position Statement dell’Endocrine Society. J Clin Endocrinol Metab 2010;95(6):2536-59.
3. Fritz KS, McKean AJS, Nelson JS et al. Analog based free testosterone methods linked to total testosterone concentrations, not free testosterone concentrations. Clin Chem 2008, 54 (3): 512-6.
4. Caputo M, Monti S. Gonadi femminili: le valutazioni ormonali. In ENDOWIKI, Lo stato dell’arte in Endocrinologia.



Attention. Please note that these items are provided only for information and are not intended as a substitute for consultation with a clinician. Patients with any specific questions about the items on this list or their individual situation should consult their clinician.